Premium
Expression of Soluble Epoxide Hydrolase (SEH) in Brains of Female Offspring of Rats Fed High Salt During Pregnancy
Author(s) -
Porter James Paul,
Johnson Clinton L,
Rogers Kristina
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1019.12
Subject(s) - epoxide hydrolase 2 , subfornical organ , offspring , endocrinology , medicine , area postrema , immunohistochemistry , basal (medicine) , hypothalamus , prenatal stress , western blot , arcuate nucleus , biology , chemistry , pregnancy , blood pressure , central nervous system , angiotensin ii , biochemistry , enzyme , genetics , gene , insulin
SEH inactivates epoxyeicosatrienoic acids (EETs) which may function in the brain to regulate blood pressure. We have previously shown that adult female rats born to mothers fed a high‐salt (HS) diet during pregnancy exhibit a hyperresponsive pressor and tachycardic response to acute stress. Microarray analysis of mRNA from brains of these HS rats suggested that SEH expression was significantly decreased. In the present investigation we used Western blot to compare the expression of SEH protein in whole brains of HS and normal salt (NS) female rats. Relative expression of SEH protein was significantly increased in the HS rats (0.97 ± 0.06 NS vs 1.72 ± 0.32 HS, n=6). Immunohistochemistry using the ABC reaction showed that SEH staining was most prominent in the medial basal region of the hypothalamus in the area of the arcuate nucleus and in regions surrounding the subfornical organ and area postrema. Morphologically the stained cells appeared to be astrocytes. These brain areas are known to be involved in the stress response and/or control of the cardiovascular system. This raises the possibility that long‐term alteration in EETs metabolism in these areas could contribute to the altered stress responsiveness programmed by prenatal high salt. Supported by a BYU Mentored Environment Grant