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Effect of chronic alcohol consumption on vitamin B1 (thiamin) uptake and metabolism by rat pancreatic acinar cells
Author(s) -
Veerapazham Thillai Sekar,
Said Hamid M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1016.3
Subject(s) - pancreas , medicine , endocrinology , thiamine , metabolism , vitamin , chemistry , acinar cell , biology
Thiamin plays a vital role in normal cellular metabolism and it deficiency leads to impairment in the function of pancreatic acinar cells. Little is known about thiamin uptake and metabolism by pancreatic acinar cells and the effect of chronic alcohol consumption on these events. We addressed these issues using cultured rat pancreatic acinar AR42J cells and primary acinar cells isolated from rat pancreas. The results showed thiamin uptake by pancreatic acinar cells to be via a specific carrier‐mediated process. Both thiamin transporters 1 & 2 (THTR‐1 and THTR‐2) were expressed in pancreatic acinar cells at the protein and mRNA levels. Chronic alcohol feeding for 4 weeks (36% calories) was found to lead to a significant reduction in carrier‐mediated thiamin uptake compared to uptake by cells from pair‐fed controls. This was associated with a marked reduction in mRNA level of THTR‐1 and THTR‐2. Similarly, chronic alcohol feeding lead to a significant reduction in mRNA level of the thiamin metabolizing enzymes thiamin pyrophophokinase, thiamin pyrophosphatase, and thiamin monophosphatase compared to pair‐fed controls. These results show for the first time that thiamin uptake by pancreatic acinar cells is carrier‐mediated. Further, chronic alcohol feeding leads to inhibition in thiamin uptake and metabolism in these cells [Supported by NIH grants DK56061‐6 and AA018071 thiamin intestine and pancreas]