Premium
Intestinal Microbiome in Transporter Null Mouse Models
Author(s) -
Engevik Melinda A,
Worrell Roger T
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1012.5
Subject(s) - bacteria , firmicutes , biology , microbiology and biotechnology , bacteroides , ileum , microbiome , bifidobacterium , bacteroides thetaiotaomicron , lachnospiraceae , lactobacillus , 16s ribosomal rna , biochemistry , genetics
A growing interest exists in the interrelation between host organism and bacteria colonization as exemplified by the NIH Human Microbiome Project. This study sought to determine the effect of altered intestinal ion transport on the luminal micobiome in mice. Ammonia is both used and produced by gastrointestinal bacteria. Changes in ammonia have been associated with shifts in bacteria species in vitro . Thus changes in transport function likely influence bacterial location and quantity. NKCC1 and cH/K‐ATPase are both implicated in epithelial cell ammonia transport. Universal, family specific, and species specific bacterial primers based on 16S ribosomal DNA were used with real time PCR to quantify bacterial populations from luminal flushes of mouse terminal ileum, ceacum, proximal and distal colon. Flushes from NKCC1 −/− and cH/K‐ATPase −/− mice showed no change in total bacteria present when compared to wild type littermates. Using family specific primers, Bacteroides and Firmicutes family members were also unchanged. Interestingly however, Bifidobacterium , a probiotic bacteria, was decreased in both ileum and ceacum of null mice, yet unchanged in colon. These data suggests that long term differences in intestinal ion transport can result in a decrease of “good” bacterial species which may increase the susceptibility of the host to intestinal dysfunction. Supported by NIH DK079979.