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PGC‐1 (Peroxissome‐proliferator activated receptor gamma co activator 1) α/β and melanoma growth: a possible energy‐mediated relation.
Author(s) -
Passos Luís Augusto,
Souza Heraldo Possolo
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1010.4
Subject(s) - tfam , mitochondrial biogenesis , cell growth , cancer cell , ppargc1a , activator (genetics) , microbiology and biotechnology , melanoma , viability assay , biology , cell , cancer , receptor , chemistry , transcription factor , cancer research , andrology , coactivator , medicine , mitochondrion , gene , genetics
The uncontrolled cell proliferation is a remarkable feature of cancer and it is known to require a large amount of energy. PGC‐1α/β were described as co activators capable of promoting mitochondria biogenesis and increase its respiratory function. Our hypothesis is that PGC‐1α/β may be over expressed in melanoma cells, increasing its capability of proliferation. We cultured normal melanocytes (Ma) and two cancer lineages (Tm1 and Tm5) and mRNA expression of PGC‐1α/β and TFAM (Mitochondrial Transcription Factor A) were analyzed by RT‐PCR. Cell growth was determined by counting in Neubawer's chamber at every 24 hours, within 5 days, using Tripan Blue exclusion to check cell viability. PGC1α mRNA was increased in Tm1 and Tm5 compared to Ma (Ma=1,23±0,24 vs Tm1=2,67±0,23 vs Tm5=3,05±0,43, p<0,01). PGC‐1β (Ma=0,47±0,02 and Tm1=0,67±0,02 vs. Tm5=0,81±0,03, p<0,05) and TFAM (Ma=0,91±0,07 e Tm1=0,89±0,03 vs. Tm5=1,17±0,06, p<0,05) expression were increased only in Tm5. Cancer cells duplication time was 24 hours whereas Ma was duplicated at 72 hours and all lineages presented 95% to 100% viability during the experiment. In conclusion, exponential growth in cancer lineages may be related to increasing energy availability mediated by PGC‐1 α/β expression. Financial support: FAPESP and DIREX‐LIM.