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The combined administration of multiple soluble factors in the repair of chronically infarcted rat myocardium
Author(s) -
Hwang Hyosook,
Kloner Robert A.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1010.3
Subject(s) - medicine , cardiology , ejection fraction , myocardial infarction , hepatocyte growth factor , cardiac function curve , growth factor , occlusion , artery , heart failure , receptor
The purpose of this study was to investigate a potential benefit of simultaneous administration of multiple soluble factors (SF) in the repair of chronically infarcted rat myocardium. Rats subjected to a permanent coronary artery occlusion (MI) received a cocktail of SF or a phosphate buffer. Four soluble factors, fibroblast growth factor‐2 (2 μg), insulin‐like growth factor‐1 (1 μg), hepatocyte growth factor (2 μg), and stromal cell‐derived factor‐1α (0.6 μg) were injected directly into the ischemic myocardium at the onset of occlusion and subsequently at 3, 7, 14, and 21 days of surgery (ip). Cardiac contractile function, infarct size and remodeling, and blood vessel density were studied at 4 weeks post surgery. Infarct size, left ventricular circumference and cavity volume, thinning ratio, and expansion index were not statistically different between groups. Treatment of SF did not alter ejection fraction, compared to control. No statistical difference in total blood vessel density in the infarct zone was observed in SF vs. control. In conclusion, our results showed that there were no enhancements in cardiac function, reductions in infarct size, improvements in remodeling, nor increases in vasculature density in SF vs. control. These results do not support the study hypothesis that the combined use of SF benefits the hearts with myocardial infarct.