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Effects of Aminoguanidine (AG) in a Rat Pancreatic Tumor Cell line
Author(s) -
Chowdhury Parimal
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1007.4
Subject(s) - chemistry , lipid peroxidation , mapk/erk pathway , cell growth , microbiology and biotechnology , blot , antioxidant , cell culture , biochemistry , signal transduction , biology , genetics , gene
Aminoguanidine is a compound that reportedly exerts both pro‐ and antioxidant effects. In animal models and in humans it was shown as an antioxidant against lipid peroxidation. The current study evaluated the direct effect of AG on rat pancreatic tumor cell. Methods AR42J cells were grown in F‐12 nutrient medium at 370C and 5% CO2. At log phase, they were separated, sub cultured for 3 days at specified dose and time with hydrogen peroxide and aminoguanidine. The cellular extracts were subjected to Western blotting employing antibodies to ERK ½ and analyzed further by immunohistochemistry. Cell proliferation lipid peroxidation assay was also conducted. Cell functional assay was conducted with CCK‐8 (10–10 M) incubated for 30 min at 370C and was assessed by the amount of amylase released in the media. Results Incubation of AR42J cells with aminoguanidine induced 3–5 fold activation of phospho ERK‐1/2. This was confirmed by immunohistochemistry with FITC conjugated antibody to ERK1/2. CCK‐stimulated amylase release was enhanced in the presence of aminoguanidine. Conclusion These data suggest that ERK‐1/2, a proliferative signal of MAPK pathway, is induced and enhanced in AR42J cells by aminoguanidine. This is coupled with enhanced cell function and proliferation suggesting that aminoguanidine acts as a pro‐oxidant in this cell line.