Premium
Ontogenetic changes of histone modifications and polymerase II binding in the promoter region of rat intestinal sugar transporter genes in vivo
Author(s) -
Suzuki Takuji,
Douard Veronique,
Mochizuki Kazuki,
Goda Toshinao,
Ferraris Ronald P
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1007.1
Subject(s) - acetylation , promoter , histone , biology , gene expression , microbiology and biotechnology , transcription (linguistics) , messenger rna , transporter , biochemistry , gene , chemistry , linguistics , philosophy
Expression of the intestinal glucose (G) transporter SGLT1 increases with age, that of the fructose (F) transporter GLUT5 does not unless its substrate is present at ≥ 14 d of age, indicating substrate‐inducible, age‐sensitive transcription. We have shown histone H3 acetylation with transcriptional activation of GLUT5 in Caco2. Here, we perfused intestines of 10 and 20 d old rats with either F or G then performed ChIP assays with antibodies against polymerase II (pol II) and acetylated histones H3 and H4 using primers targeting promoter regions 0 to −5100 bp of both genes. SGLT1 mRNA increased with age, GLUT5 increased only with F perfusion and age. Patterns of pol II binding on SGLT1 and GLUT5 promoters 0 to −200 bp correlated tightly with mRNA expression patterns. H3 acetylation in the GLUT5 and SGLT1 promoters increased with age, but acetylation in F was higher than in G between −1000 to −2000 bp only in GLUT5 suggesting total promoter sensitivity to age but specific regions dedicated to substrate induction. H3 acetylation and pol II binding did not change in the F‐responsive gene glucose‐6‐phosphatase regulated by mRNA stability. H4 acetylation did not change with age or perfusion in GLUT5 and SGLT1. Programmed age‐dependent increases in SGLT1 expression may be designed to meet hard‐wired increases in G consumption, but GLUT5 transcription increases only after weaning when the opportunity to consume F arises. (NSF722365)