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Effect of ezrin knockdown on the architecture of gastric epithelia
Author(s) -
Asano Shinji,
Tetsui Takahito,
Kobayakawa Yuka,
Tamura Atsushi,
Tsukita Sachiko,
Mukaisho Kenichi,
Hattori Takanori
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1006.2
Subject(s) - ezrin , apical membrane , secretion , parietal cell , microbiology and biotechnology , gastric glands , epithelial polarity , chemistry , epithelium , gene knockdown , gastric chief cell , gastric mucosa , cell , cytoskeleton , biology , stomach , endocrinology , gastric acid , pathology , medicine , apoptosis , biochemistry
Ezrin, an adaptor protein that cross‐links plasma membrane‐associated proteins with actin cytoskeleton, is highly concentrated on the apical surface of many epithelial cell types especially in microvilli in small intestines and stomachs. In stomachs, ezrin is predominantly expressed on apical canalicular membrane of parietal cells. The ezrin knockdown ( Vil2 kd/kd ) mice showed achlorhydria due to the impairment of membrane fusion of tubulovesicles with apical membrane. Here, we newly showed the involvement of ezrin in the architecture of gastric glands using the Vil2 kd/kd mice. The Vil2 kd/kd mice showed hypergastrinemia and hyperplasia of gastric surface epithelial cells. Atrophy was observed in the gastric glands with the number of parietal cells and chief cells being decreased, and the number of mucus neck cells being increased. The expression level of gastric proton pump was significantly decreased whereas the expression level of chloride channel CFTR was increased. These results demonstrate that ezrin is involved in the development of gastric epithelial cells and expression of transport proteins involved in gastric acid secretion.