Premium
Regional susceptibility of the small intestine to hyperthermia
Author(s) -
Phillips Neil Andre,
Novosad Veronica L,
Clanton Thomas
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1004.7
Subject(s) - hyperthermia , small intestine , ileum , jejunum , intestinal permeability , barrier function , biology , incubation , medicine , anatomy , chemistry , gastroenterology , endocrinology , biochemistry , immunology , microbiology and biotechnology
Increases in core temperature have been associated with a decline in intestinal barrier function. The loss of this barrier is believed to be an important contributor to severe heat illness. We tested the hypothesis that the small intestine displays varied changes in hyperthermia‐induced permeability along its length. We isolated and everted segments from the small intestine of adult mice, creating ~8 × 2 cm sacs from each mouse, which were then separated into heated and control groups (42 ± 0.5°C and 37 ± 0.5°C, respectively). Permeability was measured during a 90 min exposure by the accumulation of a fluorescent‐labeled dextran (4KD) that moved from the incubation media into the sacs. The data showed a significant and relatively proportional increase in permeability in the hyperthermia treated segments as a function of the distance from the anus along the length of the small intestine (P < 0.007) but no significant relationship was found in the matched group exposed to 37°C. We conclude that in the mouse, the anterior portions of the small intestine (near the jejunum) have an increased susceptibility to hyperthermia compared to posterior regions (near the ileum). This suggests that treatments targeted to protect the upper intestine may be helpful in protecting humans from severe heat illness. NHLBI 53333