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Enhanced Oxidative Burst Activity in Macrophages from Obese African American (AA) Women with Metabolic Syndrome (MetS)
Author(s) -
Colden Margaret
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1001.6
Subject(s) - oxidative stress , respiratory burst , medicine , endocrinology , inflammation , adipose tissue , monocyte , basal (medicine) , type 2 diabetes , oxidative phosphorylation , metabolic syndrome , diabetes mellitus , obesity , insulin , chemistry , immunology , biochemistry
Obese patients with MetS are at higher risk of atherosclerotic and diabetic complications since they have higher levels of free radicals and a low antioxidant capacity indicating elevated oxidative stress (Skalicky et al., 2008). AA women possess a staggering 57% higher prevalence in having MetS than AA men. To determine if obesity may affect macrophage function in AA women, basal and stimulated oxidative burst activity was measured in peripheral blood‐derived macrophages (PBDMs) from AA women who were lean/insulin‐sensitive (Lean) or obese/insulin‐resistant (Obese) using the fluorescent probe, 2′, 7′‐dichlorofluorescein. Basal oxidative burst activity was similar in Lean and Obese PBDMs compared to control human THP‐1 monocytes. Acute exposure to phorbol ester, PMA (100 nM), stimulated oxidative burst activity by 19% in THP‐1 monocytes. Stimulated oxidative burst activity in Lean PBDMs was enhanced to 317% of basal THP‐1 monocyte activity. Obese PBDMs possessed a significantly higher oxidative burst response to PMA (533% of basal THP‐1 monocytes) compared to Lean PBDMs. These data suggest that macrophage infiltration into adipose tissue may contribute to elevated oxidative stress and development of obesity‐induced inflammation in AA women with MetS. This research was supported by a MSM Center of Research Excellence Career Development Award Proj 5‐04.