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Caenorhabditis elegans grown on Mn(II) enriched diet have reduced levels of Reactive Oxygen Species (ROS) in vivo
Author(s) -
Rakariyatham Kanyasiri,
Villarreal Ponce Alvaro P.,
Trinh Kim,
Bach Cynthia,
Srinivasan Chandra
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1001.20
Subject(s) - reactive oxygen species , caenorhabditis elegans , green fluorescent protein , cytosol , divalent , in vivo , oxidative stress , antioxidant , biology , chemistry , transcription factor , microbiology and biotechnology , biochemistry , fusion protein , glutathione , enzyme , gene , recombinant dna , genetics , organic chemistry
Divalent manganese [Mn(II)] can substitute for superoxide scavenging enzyme(s) in certain unicellular organisms. In a multicellular organism , C. elegans , Mn(II) enriched diet increased this organism's tolerance towards ROS and provided lifespan extension. The molecular basis for the antioxidant‐like activity of Mn(II) is still unclear. To examine whether Mn(II) exerts this activity through the activation of the transcription factor, DAF□16, we utilized three different transgenic strains containing a DAF‐16::GFP fusion, HSP16.2::GFP fusion, and SOD3::GFP fusion. Age synchronized worms were cultivated on normal or Mn(II) supplemented diet and GFP expression was monitored. The nematodes exposed to Mn(II) exhibited a nuclear localization of DAF‐16 as opposed to residing equally between the cytosol and the nucleus under low stress (normal) condition. The expression levels of two downstream targets of DAF‐16, SOD3 and HSP16.2, were higher in worms grown on Mn(II) enriched diet in comparison to untreated controls. In vivo ROS levels measured using a fluorescent dye indicated that the worms grown on Mn(II) enriched diet had reduced levels of ROS. Collectively, our findings suggest that Mn(II) works through the DAF‐16 pathway, which leads to a reduction in ROS levels.