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Near‐Infrared LED Photobiomodulation Attenuates Hypoglycemia‐Induced Cardiotoxicity in a Rodent Model of Diabetes Mellitus
Author(s) -
Gopalakrishnan Sandeep,
Wilson Robert,
Rakita Jason,
Antoniewski Sarah,
Abroe Betsy,
Snyder Ann,
Eells Janis
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1001.18
Subject(s) - oxidative stress , diabetes mellitus , streptozotocin , diabetic cardiomyopathy , medicine , endocrinology , glutathione , cardiotoxicity , hypoglycemia , nitrotyrosine , pharmacology , chemistry , cardiomyopathy , heart failure , biochemistry , toxicity , enzyme , nitric oxide synthase , nitric oxide
Diabetes‐induced hyperglycemia disrupts mitochondrial energy metabolism and increases oxidative stress. 670 nm light‐emitting diode photobiomodulation (PBM) has been shown to protect mitochondria, attenuate oxidative damage and improve cell survival in experimental and clinical investigations. We tested the hypothesis that 670 nm photobiomodulation would attenuate hypoglycemia‐induced cardiotoxicity in a model of type I diabetes, the streptozotocin (STZ)‐treated rat. Wistar rats were randomly assigned into 3 treatment groups: Control (CON), STZ‐treated (STZ) [50 mg/kg, ip] and 670 nm NIR treated [5 min; 15 J/cm 2 ) STZ‐diabetic rats (NIR). After 98 days of diabetic hyperglycemia animals were euthanized. The heart was harvested, flash frozen and stored at −80 C until analyzed. STZ‐induced diabetes depleted total antioxidants, increased the ratio of oxidized to reduced glutathione, increased nitrotyrosine, decreased catalase, decreased thioredoxin reductase and increased caspase‐3 activity in heart tissue. NIR treatment prevented these cytotoxic actions of STZ. The results support our hypothesis that NIR photobiomodulation attenuates oxidative stress in diabetic cardiomyopathy and suggest that it has therapeutic potential as a cytoprotective strategy for the treatment of diabetes and other chronic degenerative diseases. (RGI [UWM]; BTA [State of Wisconsin]).