Premium
N‐acetylcysteine attenuates ventilator‐induced diaphragm dysfunction in rats
Author(s) -
Agten Anouk,
Maes Karen,
Smuder Ashley,
Powers Scott K,
Decramer Marc,
GayanRamirez Ghislaine
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.1001.10
Subject(s) - acetylcysteine , diaphragm (acoustics) , saline , oxidative stress , medicine , diaphragmatic breathing , anesthesia , pharmacology , chemistry , biochemistry , antioxidant , pathology , physics , alternative medicine , acoustics , loudspeaker
Controlled mechanical ventilation (CMV) results in diaphragmatic dysfunction and oxidative stress. We examined whether an anti‐oxidant, N‐acetylcysteine (NAC), would restore the redox‐balance in the diaphragm and prevent the deleterious effects of CMV. Anesthetized rats were submitted for 24h to either spontaneous breathing while receiving 150mg/kg NAC (SBNAC) or saline (SBSAL) or to CMV while receiving 150mg/kg NAC (MVNAC) or saline (MVSAL). After 24 hours, diaphragm forces were significantly lower in MVSAL compared to all groups (−21%, p<0.01). Administration of NAC completely abolished this decrease. Protein oxidation was significantly increased in MVSAL (+43%, p<0.0001) and was restored in MVNAC. Diaphragm caspase‐3 and calpain activity were significantly increased in MVSAL compared to all groups (p<0.0001). These data show that the administration of NAC was able to preserve the diaphragm from the deleterious effects of CMV. Supported by AstraZeneca Farmaceuticals and FWO Vlaanderen