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Mode of delivery and early nutrition differentially impactiIntestinal development of the neonatal piglet
Author(s) -
Radlowski Emily C.,
Wang Mei,
Monaco Marcia H.,
Nehrling Erica W.,
Tappenden Kelly A.,
Donovan Sharon M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb486
Subject(s) - colostrum , disaccharidase , ileum , jejunum , zoology , medicine , endocrinology , chemistry , ussing chamber , biology , small intestine , andrology , secretion , immunology , antibody
Route of delivery can affect microbial colonization. Herein, the hypothesis that delivery mode and early nutrition interact to modulate intestinal development was tested. Colostrum‐deprived piglets derived by vaginal (VD; n=37) or Cesarean delivery (CD; n=27) were randomized to be sow‐reared (SR) or fed formula (F) or F + fructooligosaccharides (2 g/L) and polydextrose (2 g/L) (F+P). On d7 and 14, samples of jejunum (JJ), ileum (IL) and ascending colon (AC) were collected for analysis of morphology, disaccharidase activity and electrophysiology in Ussing Chambers. JJ weight (g/kg BW) was greater in VD than CD piglets (p=0.04). IL weight increased over time (p=<0.001); there was a trend for an interaction between delivery mode and day (p=0.055). JJ mucosal weight (g/kg BW) was greater in SR than F or F+P piglets (p<0.05). Disaccharidase activities were greater in SR than F or F+P piglets (p<0.05). Transmucosal resistance was greater in JJ of SR than F or F+P piglets (p<0.05). Between d7 and d14, AC glutamine transport decreased (p<0.05) and glucose transport increased (p=0.07). Carbochal‐induced chloride secretion in the JJ was affected by diet*day and in the AC by diet*delivery. In conclusion, sow milk relative to F or F+P increased JJ mucosal weight, enzyme activity, and barrier function. In addition, prebiotics reduced chloride secretion in CD piglets to levels similar to VD piglets. (BMS Freedom to Discover Award)