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Anthocyanin protects Raw264.7 cell from ox‐LDL by decreasing CHOP expression in mRNA level
Author(s) -
Zhang yemin,
Wang Baohua,
Zhang Deling,
Ouyang Jingping
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb44
Subject(s) - chop , anthocyanin , messenger rna , microarray , chemistry , microbiology and biotechnology , endoplasmic reticulum , gene expression , biology , gene , biochemistry , food science
Cardiovascular diseases, especially artherosclerosis is responsible for the majority of deaths in the world. Ox‐LDL is an important factor in the progress of artherosclerosis. To find an effective drug or method to treat artherosclerosis, we use three antioxygens, sodium ferulate, tBHQ and anthocyanin to administrate serum starved Raw264.7 cell treating with 40mg/L ox‐LDL for 24 hours. Cytoactive was detected by MTT. We found that only anthocyanin could protect cells from ox‐LDL, and the effect was independent on concentration. CHOP (also known as growth arrest and DNA‐damage‐inducible protein GADD153) is en endoplasmic reticulum stress‐inducible protein that plays a critical role in the regulation of cell death. Gene chip (Affymetrix Mouse 4302.0 microarray) and RT‐PCR found that the mRNA of CHOP decreased in anthocyanin treating group. Therefore, we conclude that anthocyanin may protect cells by decreasing CHOP expression in mRNA level. This will provide us a new clue to cure arteriosclerosis.