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Isoflavone concentration of soy infant formula influences adipose gene expression in the neonatal piglet
Author(s) -
Schantz Brooke Blue,
Cooke Paul S.,
Donovan Sharon M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb438
Subject(s) - adipocyte , aglycone , adipose tissue , medicine , endocrinology , lipoprotein lipase , chemistry , biology , stereochemistry , glycoside
Soy isoflavones (ISO) affect adipocyte development in rodents. Currently, 25% of formula‐fed infants receive soy formulas (SF) that contain 25 mg/L aglycone ISO. Herein, the hypothesis that ISO influenced adipose development was tested. Piglets (n=74) were fed cow milk‐based formula (CMF), SF (25 mg aglycone ISO/L) or a low ISO SF (LSF; 1.1 mg aglycone ISO/L). Subcutaneous (SC) and renal adipose were collected on d 2, 7, 12 and 21. SC adipocyte number and size were measured using a Coulter Counter and gene expression was assessed by Real‐Time PCR. Weight gain was similar in all groups. Adipocyte cell size increased between d 7 and 21 (p<0.02), but no effect of diet on adipocyte number or size was observed. Diet modulated gene expression in a tissue‐specific manner. In SC adipose, PPAR‐γ expression was unaffected by diet, C/EBP‐α was higher in SF than LSF and CM, and C/EBP‐β and lipoprotein lipase (LPL) were greater (p<0.05) in SF than CM, with LSF being intermediate and not different from either. In renal adipose, C/EBP‐α was unaffected by diet, PPAR‐γ and LPL were higher in LSF than CM with SF being intermediate and not different from either, and C/EBP‐β was greater (p<0.05) in SF than CM with LSF intermediate and not different from either. Thus, compared to CMF, SF induced expression profiles consistent with adipocyte differentiation, but SF and ISO effects differed between SC and renal adipose. (Solae LLC and IL Soy Association)