Premium
The Effect of C‐Reactive Protein on Glucose Release in Primary Hepatocytes
Author(s) -
Ellis Flannery,
Pfaffenbach Kyle,
Wang Dong,
Wei Yuren,
Pagliassotti Michael
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb431
Subject(s) - insulin , medicine , endocrinology , c reactive protein , collagenase , glucose uptake , chemistry , hepatocyte , biology , in vitro , biochemistry , inflammation , enzyme
C‐reactive protein (CRP) is associated with increased risk of cardiovascular disease and can reduce insulin signaling in skeletal muscle cells. The aim of this study was to determine whether CRP impairs glucose release in primary hepatocytes. Hepatocytes were isolated from male Wistar rats (n=3) by collagenase perfusion. Primary hepatocytes were provided glucose‐free media containing 10 mM lactate, 1 mM pyruvate 0, 1 or 10 nM insulin, and 0 or 10 μg/ml CRP for 6h. Media samples were analyzed for glucose concentration and cells harvested for total protein analysis. In the absence of insulin, glucose release was not different in the absence or presence of CRP. In the presence of 1 nM insulin, CRP increased glucose release (μmol/mg protein/6h) by ~25% (14.0±1.7 vs. 11.3±1.7; mean±SD). In the presence of 10 nM insulin, CRP increased glucose release by only ~8% (13.0±2.3 vs. 12.0±0.4). This data suggests that CRP may reduce the ability of insulin to suppress glucose release in primary hepatocytes.