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Selenium Imaging in Mouse Tissues
Author(s) -
Malinouski Mikalai,
Kehr Sebastian,
Finney Lydia,
Vogt Stefan,
Gladyshev Vadim
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb413
Subject(s) - selenium , selenoprotein , knockout mouse , selenoprotein p , glutathione peroxidase , biology , olfactory bulb , pathology , chemistry , endocrinology , biochemistry , superoxide dismutase , central nervous system , receptor , oxidative stress , medicine , organic chemistry
Imaging of trace elements in cells and tissues provides an opportunity to understand their functions. Distribution of selenium under either physiological or pathophysiological conditions remains unknown due to the lack of sensitive analytical techniques. We applied X‐ray fluorescence microscopy (XFM) to map selenium distribution in various tissues of mice. High spatial resolution of XFM revealed cellular topography of selenium as well as other trace elements. Selenium distribution and content were analyzed in mouse brain, thyroid, liver, kidney, prostate, and testes. In mouse testes, selenium was primarily localized in elongating spermatids. Testes samples from mitochondrial glutathione peroxidase 4 knockout mice reduced selenium content. Removal of Selenoprotein P (SelP) also led to a significant decrease in this element in mouse testes. The highest concentration of selenium was observed in the glomerulus layer of olfactory bulb. This layer was associated with high expression of SelP. In SelP knockout olfactory bulb, selenium was decreased almost 10 fold. In kidney, selenium localized to basal membrane of proximal tubules and was not affected by SelP knockout. In conclusion, the use of XFM allowed visualization and analysis of specific cellular distribution of selenium and other trace elements in mouse tissues.