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A case‐study of fucoxanthin uptake kinetics
Author(s) -
Craft Neal E.,
ArredondoVega Francisco X.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb411
Subject(s) - fucoxanthin , metabolite , carotenoid , chemistry , human blood , urine , blood lipids , chromatography , biochemistry , medicine , cholesterol , physiology
Fucoxanthin (FX), a carotenoid in seaweed, has been reported to increase lipid oxidation in adipose tissue leading to weight loss. Little is known about FX kinetics and biochemical activity. We initiated a case study examining the uptake of FX into the blood of an adult male. Objective To monitor FX and metabolite concentrations in serum following FX consumption. Methods Blood was collected at 0, 4, 8, 24, 48, 72, 96 hrs. FX was consumed as softgels post blood draw (0 hr) and on days 1‐4. On day 0, 11mg of trans‐FX was consumed and 6.6mg on each successive day. Serum was harvested each day of blood collection and frozen. On day 4, blood samples were analyzed by HPLC using a C30 gradient separation with DAD. Based upon the results, no additional blood was collected. Results All of the major carotenoids reported in human blood plus FX and a metabolite, Fucoxanthinol (FXOH), were measured. FXOH was observed in the blood at 4hr (2.6 ng/mL) and peaked at 8hr (9.6 ng/mL) and returned to 2.2 ng/mL by 24hr after the initial FX consumption. FXOH was measured in the serum on each day (0.8‐1.1 ng/mL) but did not accumulate in the blood with each day of consumption. Blood and urine were also tested for conjugates of FX or FXOH. Discussion FX was not measurable in the blood samples. FXOH appearance and decline in serum was more rapid than reported for less polar carotenoids and it does not appear to accumulate in its endogenous form in this tissue.