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STRAW‐BASED SPECIMEN CRYOPRESERVATION & BANKING
Author(s) -
Cohen Laurent
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb355
Subject(s) - traceability , technician , workflow , sample (material) , straw , process engineering , environmental science , operations management , computer science , waste management , engineering , database , chemistry , chromatography , software engineering , electrical engineering , inorganic chemistry
When archiving serum, DNA, or other types of samples at very low temperatures for clinical trials, epidemiology or other purposes, there is a choice of methodologies and technologies. This poster compares the most common methods from the standpoints of science and economics. The parameters for consideration are the storage temperature (e.g., liquid nitrogen vs. ‐80ºC; LN2 liquid vs. vapor phase), the type of container (e.g., upright vs. chest freezer), and packaging (e.g., vials vs. straws) used for the repository. Different configurations were assessed according to the following criteria: "Quality" preservation as measured by stability of markers and cell survival rates, "Safety" assessed from the standpoint of bio‐containment and cross‐contamination risk, "Efficiency" quantified by the number of aliquots per container unit, "Traceability" of the samples throughout the chain of custody, as well as the level of "workflow automation" and general "ease‐of‐use". A comprehensive cost model was developed to evaluate the overall cost per sample for any storage configuration. The model includes capital equipment, maintenance, "fuel" (electricity or LN2), technician labor, and additional air conditioning required. This poster presents the results and outlines the scientific and economic benefits of a straw‐based, integrated and automated solution.