Premium
CIB1 Is Important For Tumor‐Induced Pathological Angiogenesis
Author(s) -
Zayed Mohamed Adel,
Yuan Weiping,
Chalothorn Dan,
Barry William,
Faber James,
Parise Leslie
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb336
Subject(s) - angiogenesis , pathological , pathology , cancer research , cd31 , neovascularization , perfusion , melanoma , medicine
Pathological angiogenesis contributes to various malignant, ocular, and inflammatory disorders, emphasizing the need to further understand it on a molecular level. We previously demonstrated that CIB1 is essential for endothelial cell (EC) function and ischemia‐induced pathological angiogenesis. We therefore hypothesized that CIB1‐KO mice may also have a defect in tumor‐induced angiogenesis. To test this hypothesis, either B16 melanoma or Lewis lung carcinoma cells were xenografted in age‐matched wildtype and CIB1‐KO mice. For 14 days, growth and intra‐tumoral perfusion were monitored non‐invasively with a scanning laser‐Doppler perfusion imager. Tumors that developed in CIB1‐KO mice were grossly necrotic in appearance, demonstrated less perfusion, and were smaller in weight and volume (p < 0.05). Biopsies obtained from melanoma tumors in CIB1‐KO mice demonstrated significantly reduced intra‐tumoral microvessel density (p = 0.002). Biopsies also showed a trend towards more tumor necrosis and hemorrhage, but this was not statistically significant (p = 0.06, and p = 0.13, respectively). Preliminary microarray data suggests that CIB1‐KO ECs have altered expression of various pro‐ and anti‐angiogenic genes. These data demonstrate that CIB1 plays an important role in tumor‐induced pathological angiogenesis. This work was supported by NIH2‐P01‐HL45100 (LVP) and NIH HL‐62584 (JEF).