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Oxidized L‐A‐phosphatidylcholine B‐arachidonoyl‐gamma‐palmitoyl (oxPAPC) induces Increased Synthesis of mRNA and protein expression for IL‐6 in Human Aortic Endothelial Cells
Author(s) -
Dvoracek Lucas A,
Kreisberg Jeffrey I,
mckinney jordan m,
schmid gabrielle,
francis Amanda d,
francis Amanda d,
lee Huey Miin,
detrick melinda s,
kreisberg robert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb264
Subject(s) - inflammation , messenger rna , chemistry , microbiology and biotechnology , medicine , immunology , biology , gene , biochemistry
Oxidized L‐A‐phosphatidylcholine B‐arachidonoyl‐gamma‐palmitoyl (oxPAPC) induces Increased Synthesis of mRNA and protein expression for IL‐6 in Human Aortic Endothelial Cells Lucas A. Dvoracek 1 , Jeffrey I. Kreisberg 1 , Jordan McKinney 1 , Gabrielle Schmid 1 , Amanda D. Francis 1 , Huey Miin Lee, Ph.D. 2 , Melinda S. Detrick 1 , and Robert Kreisberg 1 1 West Liberty State College, West Liberty, West Virginia 26074 2 West Virginia University, Morgantown, WV 26506‐6330 L‐A‐phosphatidylcholine B‐arachidonoyl‐gamma‐palmitoyl (PAPC), a bio‐active component of low density lipoprotein, can become oxidized in the blood. OxPAPC may play a role in triggering an inflammatory reaction at the endothelial cell level. Atherosclerosis, the build‐up of plaque in the vascular wall, may be exacerbated by chronic inflammation initiated by the presence of oxPAPC. In this study, human aortic endothelial cells were treated for 6h with 50μg/mL of PAPC, oxPAPC, or neither. Cellular lysates were collected and treated for mRNA isolation. Transcripts were amplified by RT‐qPCR using primers specific for Interleukin‐6 (IL‐6). Interleukin‐6 has been identified as a potentially sensitive indicator of cardiovascular disease. Data from the RT‐qPCR technique suggests a 23‐fold up‐regulation of IL‐6 mRNA for cells treated with oxPAPC over both PAPC‐treated and No Treatment groups. This publication was made possible by NIH Grant Number 2P20 RR016477 from the IDeA Networks of Biomedical Research Excellence Program of the National Center for Research Resources, WVNASA, and WVEPSCoR SURE