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Characterization of Human Cervical Mucous Proteome
Author(s) -
Panicker Gitika,
Ye Yiming,
Wang Dongxia,
Unger Elizabeth R
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb241
Subject(s) - proteome , biomarker discovery , biomarker , cervical cancer , glycosylation , biology , blood proteins , computational biology , chemistry , cancer , proteomics , bioinformatics , biochemistry , gene , genetics
Cervical cancer is among the most common cancers in women worldwide. Discovery of biomarkers for early detection of cervical cancer would improve current screening practices and reduce the burden of disease. In this study, we report characterization of the human cervical mucous proteome as the first step towards protein biomarker discovery. Proteins extracted from pooled mucous were characterized using 1‐DE and 2‐DE followed by LC/MS. Post‐translational modifications like phosphorylation and glycosylation of proteins were analyzed using fluorescent stains. A total of 107 unique proteins were identified, with plasma proteins being most abundant. These proteins represented major functional categories of metabolism, immune response, and cellular transport. Removal of high molecular weight abundant proteins by immunoaffinity purification did not significantly increase the number of protein spots resolved. Each method identified some unique proteins, indicating the advantage of utilizing multiple techniques in sample preparation, fractionation, and analysis. Thus, profiling cervical mucous proteins can further our understanding of the proteome and its potential as biomarkers.