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Shotgun Proteomics Identifies Protein Biomarkers Specific for Acute Renal Transplant Rejection
Author(s) -
Sigdel Tara,
Kaushal Amit,
Gritsenko Marina,
Norbeck Angela,
Xiao Wenzhong,
Qian WeiJun,
Camp David G.,
Sarwal Minnie M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb239
Subject(s) - biomarker , biomarker discovery , urinary system , tamm–horsfall protein , shotgun proteomics , proteomics , urine , medicine , proteinuria , pedf , kidney , chemistry , biochemistry , gene , vegf receptors
Background Proteomics can help in biomarker discovery for different diseases. Since, acute rejection (AR) remains the primary risk factor for the renal transplant outcome; development of non‐invasive biomarkers for AR diagnosis is important. Materials and Method: We analyzed 82 urine samples that included patients with biopsy proven AR and stable grafts (STA), nonspecific proteinuria (NS), and healthy controls (HC). The samples were fractionated and processed by LC‐MS. Protein‐level spectral counts were obtained by which were used to get quantitative information. ELISA assays on UMOD, pigment epithelium‐derived factor (PEDF) and CD44 were performed to verify the trueness of the discovery. Results A total of 1446 unique urinary proteins were identified including 22 AR specific proteins a number of which were involved in antigen presentation and complement system pathways. We performed ELISA assay on UMOD, PEDF and CD44 on an independent set of urine samples and demonstrated that the observation from LCMS experiment was true and these proteins can serve as potential biomarkers to discriminate AR urine from STA, NS and HC. Conclusion A number of potentially AR specific biomarkers were identified. The validation of these potential biomarkers will offer a clinically applicable, non‐invasive urinary diagnostic assay for AR.