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Long‐time influence of copper ions on the wine yeast populations increases number of resistant morphs
Author(s) -
Shatirishvili Aivengo,
Machavariani Nino,
Zarnadze Tea
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb237
Subject(s) - wine , biology , copper , yeast , population , resistance (ecology) , pesticide , ecology , food science , genetics , chemistry , organic chemistry , demography , sociology
Natural populations of the wine yeast (Sacch. cerecisiae var. vini) have been exposed to copper‐containing pesticides for a long time. The strains belonging to two different populations of the wine yeast were investigated on their resistance. One of the populations was 'Rqatsiteli', that had continuously been exposed to copper ions for many years, but the other ('Izabela') had never been treated with the pesticides. The strains were isolated from the wine sediment. 250 strains from each population were analyzed and 10 of them belonged to micropopulations. The 'Rqatsiteli' strains were found to be heterogeneous: when growing on the medium supplemented with 1‐7mM copper ions, they quitted growing in asynchronous manner. Existence of three morphs was stated: 'sensitive' ‐ 31.6%, 'mild' ‐ 38.8% and 'resistant" ‐ 29.6%. 'Izabela' population expressed less resistance when compared to "Rqatsiteli' strains and varied within the range of 1‐5 mM with only two morphs among them: 'sensitive' ‐ 63.6% and mild ‐ 36.4%. Genetic analysis in 10 strains for each population was performed. The strains of 'resistant' morphs contained two active resistance‐determining loci while the others ‐ just one. The both populations were polymorphic if evaluated by the trait under study. Driven by natural selection, number of the strains resistant to copper ions were increased.