Premium
The N‐terminal region of BRG1 contains protein interaction domains that are required for SWI/SNF activity
Author(s) -
Trotter Kevin W.,
Archer Trevor K.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb194
Subject(s) - swi/snf , chromatin remodeling , microbiology and biotechnology , transcription factor , chromatin , chromatin immunoprecipitation , chromatin structure remodeling (rsc) complex , biology , nuclear protein , transcriptional regulation , nuclear receptor , genetics , dna , promoter , gene , gene expression
The packaging of eukaryotic DNA into chromatin creates an impediment to the process of transcription by hindering binding of essential factors required for transcriptional initiation. BRG1, the central ATPase of the human SWI/SNF‐BAF complex, has been shown to be a critical factor for the functional activity of nuclear receptor complexes. Studies using BRG1 truncation and deletion mutants suggest the chromatin remodeling protein contains functional motifs important for transcriptional regulation. To further examine these conserved domains we performed a yeast‐two hybrid study using baits spanning the BRG1 N‐terminus to identify proteins that may be required for SWI/SNF activity. Several proteins were found to associate within the N‐terminal region of BRG1 including BRD7, a regulator of cell cycle progression, CBX5, which is involved in heterochromatin maintenance, and transcriptional regulators ARA160 and SSXT, as well as proteins with identified roles in apoptosis, replication and DNA repair. To verify these potential BRG1 interacting proteins we performed co‐immunoprecipitation studies using various human cell lines. Subsequently, we will perform siRNA knockdown assays to determine if these BRG1 associating proteins are required for SWI/SNF activity including nuclear receptor‐mediated transcriptional activation from endogenous chromatin loci.