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The Effects of the Herbal Enzyme Bromelain Against Breast Cancer Cell Line GI101A
Author(s) -
Paroulek Alexandra F,
Jaffe Mark,
Rathinavelu Appu
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb18
Subject(s) - bromelain , apoptosis , cytotoxicity , cancer , proteolytic enzymes , cell , immune system , cell culture , cancer research , medicine , pharmacology , biology , enzyme , immunology , biochemistry , in vitro , genetics , protease
Bromelain is a proteinase derived from the stem of pineapple and has been studied for its anti‐inflammatory, antithrombotic, and antimetastatic properties. Bromelain has also been known to significantly reduce local tumor growth and to raise the impaired cytotoxicity of monocytes in the immune system against tumor cells. The goal of this project is to advance the mechanistic knowledge of herbal remedies and to confirm the already known antimetastatic properties of Bromelain. The MTS assay method was used 24 hours after Bromelain treatment to detect the cell death. The data show that after 1 μM of Bromelain treatment, the population of GI101A cells is significantly reduced by up to 70%. Using the M30 Apoptosense assay, levels of the protein cytokeratin 18 (CK18) were measured to detect any apoptotic activity. After 10 μL of Bromelain treatment, CK18 levels increased and a large number of apoptotic cell bodies were observed. The antitumor effects of Bromelain are mainly involved in cancer cell division by the induction of apoptosis.