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ISOLATION AND CHARACTERIZATION OF BIORESPONSIVE RENAL CELLS FROM HUMAN AND LARGE MAMMAL WITH CHRONIC RENAL FAILURE
Author(s) -
Presnell Sharon C,
Bruce Andrew,
Wallace Shay M,
Choudhury Sumana,
Kelley Russell W,
Tatsumi Patricia,
Werdin Eric,
Rivera Elias,
Merricks Elizabeth,
Nichols Timothy C,
Jennette J. Charles,
Jayo Manuel J,
Bertram Timothy A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb143
Subject(s) - kidney disease , erythropoietin , medicine , immune system , dialysis , immunology , pathology
Chronic kidney disease (CKD) is a global public health problem; U.S. patients on dialysis awaiting organ transplant more than doubled between 1991 and 2004. To avoid an immune response to implanted cells, functional autologous cells would be preferred components of regenerative medicine therapies for CKD; however, evidentiary support for autologous sourcing of therapeutically‐relevant cells from CKD patients and large animal models is lacking. Fresh kidney tissue (CKD and non‐CKD) was obtained from porcine and human subjects. CKD was confirmed by serology and histopathology. Tissue dissociation and cell isolation methods developed with non‐CKD tissue were employed to isolate and propagate cells from CKD tissue and yielded tubular cells and erythropoietin (EPO)‐expressing cells. Comparative in vitro studies demonstrated expression of megalin:cubilin and receptor‐mediated uptake of fluorescein‐conjugated albumin in tubular cell cultures from both CKD and non‐CKD tissues. Cells expressing erythropoietin (EPO) were present in both CKD and non‐CKD tissues and could be isolated and expanded with retention of oxygen‐responsive, HIF1‐alpha‐driven EPO expression. Taken together, these results suggest that autologous sourcing of at least two therapeutically‐relevant cell populations is feasible in advanced CKD.