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Mice presenting skeletal muscle hypertrophic phenotype driven by mIGF‐1 over‐expression have improved carbohydrate metabolism and insulin sensitivity.
Author(s) -
Christoffolete Marcelo Augusto,
OliveiraCosta Monique,
Okamoto Maristela M.,
Turri Antonio,
Lohmann Tania Ochi,
Ribeiro Miriam Oliveira,
Machado Ubiratan Fabres,
Musarò Antonio,
Moriscot Anselmo Sigari
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb109
Subject(s) - endocrinology , medicine , carbohydrate metabolism , respiratory quotient , skeletal muscle , carbohydrate , metabolism , insulin , chemistry , insulin resistance , glucose uptake , insulin sensitivity , biology
Objective In this study, we aim to characterize the metabolic profile of mice presenting higher muscle mass driven by mIGF‐1, a muscle specific trophic factor. Methods WT and mIGF‐1 TG mice were obtained from our inbreed colony. O2 consumption and CO2 generation was determined in an open circuit respirometer. kITT (%/min) was determined by calculating the slope of the linear regression of neperian logarithm of glycemia values over time in a standard 15 min ivITT test using 0.125U/Kg of insulin. The results were expressed as mean±SEM. Results As expected, 3 months old TG mice were heavier than age matched WT mice (33.3 + 0.3 vs 29.7 + 0.4 g, p<0.0001, n=9‐15, respectively). TG mice have a higher non‐protein respiratory quotient then WT (0.9 + 0.03 vs 0.74 + 0.02, p<0.001, n=7, respectively). This data suggested a preference for carbohydrate as major fuel source in TG mice. TG mice have a higher rate of glucose disposal when compared to WT (3.25 + 0.14 vs 2.39 + 0.03 %/min, p<0.01, n=3‐5, respectively). These data altogether reveals a shift in metabolism towards higher carbohydrate utilization and improved insulin sensitivity in mIGF‐1 TG mice.