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Intrauterine growth restriction inhibits cell proliferation within the subventricular zone and white matter of rat offspring
Author(s) -
Campbell Leigh Rhodes,
Zheng Baoying,
Ojeda Norma B,
Royals Thomas P,
Rhodes Philip G,
Pang Yi,
Alexander Barbara T
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb103
Subject(s) - subventricular zone , intrauterine growth restriction , offspring , white matter , h&e stain , medicine , endocrinology , biology , immunohistochemistry , andrology , fetus , pregnancy , neural stem cell , stem cell , microbiology and biotechnology , genetics , radiology , magnetic resonance imaging
Our laboratory has developed a unique rat model of placental insufficiency that results in intrauterine growth restriction (IUGR). Many studies demonstrate an association of IUGR with atypical white matter development and abnormal neurologic outcome. We hypothesize that IUGR will affect cerebral development, and this insult will result in histopathologic changes. At postnatal day 6 (P6) and 14 (P14), rat pups were sacrificed. The brains were fixed with 4% paraformaldehyde and frozen sections prepared. There was no evidence of brain damage in the basic hematoxylin and eosin staining of IUGR rat pups at P6 and P14. However, immunohistochemistry with Ki‐67 demonstrated a significant decrease in cell proliferation within the subventricular zone (SVZ) and the surrounding white matter (719.7±119.7 vs. 339.4±55.3 cells/mm 2 ; female control vs. female IUGR, respectively, P<0.05), but not in the cortex of P6 female IUGR rat pups. The decreased number of Ki‐67 + cells in the SVZ and white matter suggests possible damage or delay in brain development of the female IUGR rat.