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Alcohol Intoxication Inhibits Sca‐1 Expression by Bone Marrow‐Derived Gr‐1+ Cells During Bacteremia
Author(s) -
Melvan John Nicholas,
Raasch Caroline,
Bagby Gregory J,
Nelson Steve,
Zhang Ping
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.lb101
Subject(s) - bacteremia , bone marrow , flow cytometry , haematopoiesis , intraperitoneal injection , alcohol , immune system , granulocyte , saline , immunology , medicine , ethanol , sepsis , alcohol intoxication , pharmacology , biology , stem cell , microbiology and biotechnology , biochemistry , antibiotics , poison control , environmental health , injury prevention
We have shown that enhanced Sca‐1 expression by lineage negative hematopoietic precursors is a key component of the granulopoietic response to bacterial infection, which is impaired by alcohol intoxication. Currently, no knowledge exists about the effects of alcohol on the Sca‐1 response in cells committed to the granulocyte lineage (Gr‐1 + ). This study investigated Sca‐1 expression by Gr‐1 + cells during bacteremia and the effect of alcohol on this response. Acute alcohol intoxication was induced via intraperitoneal (i.p.) injection of 20% alcohol (5g ethanol/kg) in Balb/c mice. Thirty minutes after i.p. injection, bacteremia was initiated by intravenous injection of E. coli (5x10 7 CFU in 100 µL saline/mouse). Animals were sacrificed 12 and 24hrs thereafter. Bone marrow cells were analyzed by flow cytometry. Bacteremia increased the percentage of Gr‐1 low and Gr‐1 high cells coexpressing Sca‐1 at both time points. Alcohol intoxication suppressed these responses. These data show that alcohol suppresses Sca‐1 expression in cells committed to granulocyte lineage development during bacteremia, which may serve as a mechanism underlying alcohol induced immune suppression.