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Cloning and Expression of Aquaporin in the Antennal Gland of Crayfish, Procambarus Clarkii
Author(s) -
Gao Yang,
Gao Yongping,
Wheatly Michele,
Krane Carissa
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.998.6
Subject(s) - crayfish , biology , hepatopancreas , procambarus clarkii , messenger rna , aquaporin , complementary dna , hemolymph , moulting , microbiology and biotechnology , anatomy , gene , biochemistry , fishery , ecology , larva
Aquaporins (AQPs) , water transport proteins, have been cloned from a wide variety of species, however, little is known in crustaceans. The objective of this study is to clone the AQP from crayfish and examine the mRNA expression, both temporally (molting cycle) and spatially (tissue‐specific). A pair of degenerate primers was used in amplifying a 367bp fragment of cDNA from crayfish antennal gland. The deduced amino acid sequence showed homology with AQPs from mosquito (54%), drosophila (52%), rat (42%) and human (43%). The AQP mRNA expression in the antennal gland was compared with that of other epithelia tissues: gill and hepatopancreas and non‐epithelia tissues: cardiac muscle and tail muscle, by using real‐time PCR. Compared with antennal gland, the expression was lower in gill and higher in cardiac muscle and hepatopancreas respectively. No significant difference was found in tail muscle. Also, the relative mRNA expression of AQP in antennal gland is lower in the premolt when compared to inter‐ and post‐ molt stages. These preliminary observations suggest that crayfish AQP does play an important role in transferring water, regulating nutrients and salt conservation in multiple tissues and the molting stages. Future study will be focused on the expression of this aquaporin in the different tissues of crayfish during molting. (Supported by Grants IBN 0445202 to MGW, YG and CMG).