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Short‐term insulin infusion alone has negative effect on skin protein mass in fasted pigs
Author(s) -
Tuvdendorj Demidmaa,
Børsheim Elisabet,
Sharp Carwyn P,
Zhang Xiaojun,
Aarsland Asle,
Herndorn David N,
Chinkes David L,
Wolfe Robert R
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.991.22
Subject(s) - insulin , medicine , endocrinology , hyperinsulinemia , phenylalanine , intracellular , amino acid , chemistry , insulin resistance , biochemistry
The effect of insulin (INS) on skin protein turnover (fractional synthesis and breakdown rates, FSR and FBR, respectively) in pigs is presented. Methods Pigs were divided into 4 groups: Control (CNT), Insulin (INS), Amino Acid (AA) and INS+AA. L‐[ring‐ 2 H 5 ]‐phenylalanine (Phe), INS and/or AAs were infused during the study. FBR was estimated from enrichment ratio of arterial Phe to intracellular free Phe. Results Plasma INS was increased in INS and INS+AA ( p <0.05). Plasma glucose was constant in all groups. In INS group tissue intracellular branched and essential AA pools were lower than in other groups although did not reach statistical significance. The ratio of Phe inward transport to intracellular breakdown increased in AA ( p <0.05) and INS+AA groups ( p= 0.05) compared to CNT. Hyperinsulinemia increased pig skin protein FSR and FBR, decreasing Net Balance (NB) (Table 1). Conclusion Ins infusion alone has a negative effect on protein mass in normal fasted pig skin because of insufficient AA availability. Fundings: NIH AR0490380‐05, Shriners 8490.