Premium
The anabolic effect of insulin is dependent on its ability to increase blood flow and muscle perfusion in human subjects
Author(s) -
Timmerman Kyle L,
Lee Jessica L,
Dhanani Shaheen,
Dreyer Hans C,
Glynn Erin L,
Fry Chris S,
Drummond Micah J,
Rasmussen Blake B,
Volpi Elena
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.991.21
Subject(s) - insulin , medicine , endocrinology , basal (medicine) , anabolism , perfusion , skeletal muscle , blood flow , glucose uptake
Insulin‐induced blood flow has been shown to correlate positively with insulin's ability to enhance muscle protein synthesis. Thus, we hypothesized that pharmacological inhibition of insulin‐induced vasodilation would reduce the anabolic response of muscle proteins to insulin. Skeletal muscle fractional synthetic rate (FSR), net balance, blood flow (indocyanine green), and muscle perfusion (contrast enhanced ultrasound) were measured in 10 healthy, glucose tolerant subjects (31±2 yrs) in the basal state and during administration of insulin alone (N= 5) or insulin plus the NO synthase inhibitor N G ‐monomethyl‐L‐arginine (L‐NMMA) (N= 5). There were no differences in the basal period. FSR increased significantly (P<0.05) in subjects receiving insulin alone (basal: 0.059%/h vs. insulin: 0.079%/h) compared to subjects receiving insulin plus L‐NMMA (basal: 0.056%/h vs. insulin+ L‐NMMA: 0.045%/h). Those receiving insulin alone experienced a significantly (P<0.05) greater percent change from baseline (54.90%) in muscle perfusion than subjects receiving insulin + L‐NMMA (3.14%). There was also a trend (P=0.09) for increased blood flow in subjects receiving insulin alone. These preliminary data suggest that the ability of insulin to stimulate blood flow and muscle perfusion is necessary for insulin to stimulate muscle protein synthesis in healthy, young adults. Funded by NIH R01 AG018311 and UTMB Claude D. Pepper Older Americans Independence Center Grant #P30 AG024832.