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Tumor necrosis factor‐alpha production by peripheral blood dendritic cells in post menopausal type 2 diabetic women
Author(s) -
Blank Sally Elliott,
Johnson Emily Carolyn,
Weeks Debbie,
Wysham Carol H.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.990.7
Subject(s) - glycemic , medicine , tumor necrosis factor alpha , type 2 diabetes , endocrinology , diabetes mellitus
The objective was to examine if tumor necrosis factor‐alpha (TNF‐α) production by subsets of blood dendritic cells (DC), plasmacytoid (pDC) and myeloid (mDC), is altered in post‐menopausal women with type 2 diabetes (T2D).Women (n=21) were recruited from a local sample enrolled in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) clinical trial. Plasma glucose and TNF‐α were determined by amperometry and ELISA, respectively. Intracellular TNF‐α was analyzed by flow cytometry. T2D had greater (p<0.05) BMI, plasma glucose and TNF‐α vs healthy women (n=21). Poor glycemic control (HA1c > 7%) was present in 67% of T2D. Age and glycemic control (GLYC) effects, examined by ANOVA, indicated that plasma TNF‐α was greater in T2D with poor GLYC, independent of age (p=0.004). An interaction existed between age and GLYC (p=0.041) with a trend (p=0.07) for lower mDC TNF‐α in older women (age > 60 yr) with poor GLYC. T2D with plasma TNF‐α >15pg/mL had fewer stimulated mDCs (%) expressing TNF‐α vs healthy women (p=0.045). The findings support the hypothesis that TNF‐α production by DCs is modified by glycemic control in women with T2D. Support by McLeod Endowment Fund, WSU College of Pharmacy.