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Absorption and biosynthesis of D‐chiro‐inositol in mice
Author(s) -
Gopalan Chaya,
Lin Xiaobo,
Ma Lina,
Ostlund Richard
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.990.2
Subject(s) - urine , chemistry , medicine , endocrinology , inositol , deuterium , receptor , physics , quantum mechanics
D‐chiro‐inositol (DCI) is known to possess insulin‐like properties. Mice were fed with either regular chow (control group) or DCI‐free diet over a five‐week period. The levels of DCI declined significantly in plasma (1.91 ± 0.17 to 0.056 ± 0.0 μM), stool (20.2 ± 6 to 0.147 ± 0.05 nmoles) and urine (27.39 ± 8.2 to 0.88 ± 0.09 μM) in mice fed with DCI‐free diet at the five‐week period compared to control (plasma 2.47 ± 0.3 to 2.41 ± 0.29 μM; stool 25.7 ± 8.67 to 31.6 ± 9.7 nmoles and urine 39.9 ± 10.3 to 51.5 ± 6.8 µM). Whether DCI could be synthesized endogenously was tested next in the mice maintained on DCI‐free diet for 10 weeks where deuterated water was administered (20 % w/w deuterium oxide) for one week followed by a 24‐hour urine collection. The concentration of deuterium oxide was 8.7 + 0.52 % but there was no natural or deuterated DCI (<0.0067 nmoles/mL) in urine. Instead, there was deuterated myo‐inositol (dMI; 34.4 + 2.8 %) synthesis. To test whether MI could be converted to DCI in mice treated with DCI‐free diet, dMI (1mg MI‐d6) was injected intraperitoneally and urine was collected. Although MI‐d6 constituted 38.7+11.5 % of urinary MI, there was no natural or deuterated DCI present (<0.0067 nmoles/mL). Thus, DCI appears to be derived solely from the diet and may represent additional nutritional factor related to insulin action.