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Vascular Remodeling of Mesenteric Resistance Arteries in Diabetic Mice
Author(s) -
Souza Flavia Moreira,
Katz Paige S.,
Stewart James A.,
Vassallo Dalton V.,
Lucchesi Pamela A.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.990.19
Subject(s) - electrical impedance myography , medicine , mesenteric arteries , endocrinology , zymography , insulin resistance , diabetes mellitus , compliance (psychology) , anatomy , matrix metalloproteinase , artery , cardiology , vasodilation , psychology , social psychology
Aims Little is known about Type 2 diabetes‐induced structural remodeling of mesenteric resistance arteries. This study compared structural differences in small mesenteric resistance arteries in diabetic (db/db) and control (Db/db) mice. Methods Vessels were isolated from 16wk old mice and structural properties were assessed by pressure myography. Mean arterial pressure (MAP) was measured in vivo by telemetry. Western blot analysis and gelatin zymography were used to assess the expression and activity of matrix regulatory proteins. Results MAP was similar between control and db/db mice. Fasting blood glucose levels were higher in db/db mice (505±28 mg/dl) vs. control (115±10, p<0.001). The lumen diameter (121±5.5 μm vs. 77.8±5.5, p<0.001 at 75 mmHg) and media cross‐sectional area were significantly increased in db/db compared to control, respectively, suggesting hypertrophic outward remodeling. Cross‐sectional compliance was significantly larger in the diabetic arteries at 50 and 75 mmHg. The stress‐strain curve was shifted to the right in mesenteric arteries from diabetic mice compared with controls. The expression of the matrix regulatory proteins MMP‐9, MMP‐12, TIMP‐1, TIMP‐2, TGF‐β and PAI‐1 were increased in db/db arteries, while MMP‐2 activity was decreased. Conclusions These data suggest that diabetic mesenteric resistance arteries undergo hypertrophic outward remodeling, increased vessel compliance and reduced stiffness that was associated with ECM turnover secondary to an imbalance between pro and anti‐fibrotic factors. Supported by COBRE P20RR18766 and HL56046 (PAL) and CAPES (DV).