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No effect of diabetes on intramyocellular diglyceride accumulation in human skeletal muscle
Author(s) -
Anastasiou Costas A,
Kavouras Stavros A,
Lentzas Yannis,
Gova Afrodite,
Sidossis Labros S,
Melidonis Andreas
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.990.10
Subject(s) - medicine , endocrinology , insulin resistance , diabetes mellitus , type 2 diabetes , glycemic , obesity , chemistry
Intramyocellular lipids may be linked to insulin resistance. We aimed to investigate the effect of adiposity and diabetes on intramyocellular diglycerides (DG) and triglycerides (TG). The study participants were stratified according to the presence of diabetes and obesity into three groups: non‐diabetic, obese (nDiab‐Ob, n =19), diabetic, non‐obese (Diab‐nOb, n =4) and diabetic, obese subjects (Diab‐Ob, n =11). Intramyocellular lipids were determined in biopsies from the vastus lateralis under fasting. Glycemic control and insulin resistance were assessed by an oral glucose tolerance test and the homeostatic model. Diab‐Ob had significantly higher intramyocellular TG, compared to nDiab‐Ob (66.7±23.8 vs. 18.4±4.4 nmol/mg dry tissue, p<0.05), while no difference was observed between nDiab‐Ob and Diab‐nOb. DG levels were not significantly different among the study groups (1.7±0.3, 1.1±0.2 and 1.9±0.7 nmol/mg dry tissue for nDiab‐Ob, Diab‐nOb and Diab‐Ob respectively). Monounsaturated fatty acids were the major constituent of TG in all groups, while DG contained mainly saturated fatty acids. No correlation was found between intramyocellular TG or DG levels and insulin resistance. A modest correlation was found between monounsaturated fatty acids in the TG and the area under the glucose curve (R=0.383, p<0.05). Our data do not provide evidence for a major role of intramyocellular DG in insulin resistance.