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Effect of previous inflammation on the susceptibility of the colon to subsequent physical restraint stress‐induced changes
Author(s) -
Santiago Olga I.,
Cruz Mirella L.,
Cuevas Marielly,
Isidro Raimond A.,
Appleyard Caroline B.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.986.3
Subject(s) - inflammation , colitis , immune system , lactobacillus , medicine , immunology , cytokine , gastroenterology , bacteria , biology , genetics
Stress can influence the systemic and local immune systems of the intestine, and we have previously shown the susceptibility of the colon to psychological water avoidance stress. Aim: Determine the effect of previous intestinal inflammation on restraint stress‐induced changes in the colon. Methods Four groups of female rats were used: normal; control‐RS (restraint stress ‐ stress alone); colitis, (trinitrobenzene sulfonic acid 30 mg in 50% ETOH ic) which was allowed to heal for 2 weeks); and colitis‐RS (colitis, then RS 2 weeks later). RS was induced by placing the animals in a wire restrainer for 3hrs, during 3 consecutive days. The colons were examined for damage and levels of IL‐10, IL‐4 and IFN gamma, colonic mast cells and bacterial levels in MacConkey, blood agar and lactobacillus media were measured. Results Prior inflammation caused increases in colonic propulsive activity, mast cells, macroscopic and microscopic damage in the animals exposed to RS. There were lower numbers of lactobacillus. No significant changes in total bacterial load or cytokine levels were found. Conclusion Prior inflammation increases the susceptibility of the colon to respond to physical stress.