Premium
Resuscitation strategy alters response to hemorrhage
Author(s) -
Makley Amy Teres,
Friend Lou Ann,
Goodman Michael,
Blanchard John,
Lentsch Alex,
Pritts Timothy
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.977.6
Subject(s) - resuscitation , medicine , myeloperoxidase , shock (circulatory) , hemorrhagic shock , anesthesia , inflammation , blood pressure , femoral artery , surgery
Objective Hemorrhagic shock is associated with a systemic inflammatory response. Two common resuscitation strategies are crystalloid and blood component therapy. The effect of these strategies on inflammation is not fully understood. We hypothesized that crystalloid would exacerbate inflammation after hemorrhagic shock. Methods Mice underwent pressure controlled hemorrhage via femoral artery cannulation and resuscitation with whole blood (WB) or Ringers lactate (LR). Survival and fluid requirements were determined. Tissue myeloperoxidase (MPO) levels were analyzed. Results Resuscitation with LR resulted in less survival at 7 days vs WB or sham (Fig A). LR mice required more fluid (Fig B) and exhibited higher MPO activity in jejunum, but not liver or kidney (Fig C). Conclusions LR resuscitation led to increased fluid requirements, jejunal MPO and mortality compared to WB. These results are critical to understanding potential benefits of blood resuscitation following trauma. Supported by a grant from the Henry Jackson Foundation