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Synbiotic modulation of cytokine secretion in ulcerative colitis colonic biopsies
Author(s) -
Urdaneta Elena,
Alfaro Maria,
Jauregui Patricia,
Zozaya Jose Manuel,
Arin Antxon,
Santidrian Santiago,
Marzo Florencio
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.977.5
Subject(s) - ulcerative colitis , proinflammatory cytokine , immune system , immunology , cytokine , medicine , bifidobacterium bifidum , lactobacillus casei , interleukin 10 , bifidobacterium , inflammation , lactobacillus , biology , disease , bacteria , fermentation , food science , genetics
Ulcerative colitis (UC) consists of an aberrant local immune response to components of the bacterial microflora. There is also evidence that mucosal immune cells actively interact with non‐immune cells to promote tissue damage, and that cytokines are essential mediators of this cross‐talk. Functional foods, with beneficial effects for human health, such as a synbiotic, composed of Lactobacillus casei (Lc) and Bifidobacterium bifidum (Bb) (108 cfu/mL), and sphingomyelin (0.05%), could modulate this inflammatory disease. The aim of this study was to investigate pro and anti‐inflammatory cytokine production in colonic biopsies from UC patients and healthy volunteers examining this synbiotic effect. Patients were asked to give written consent to take part in this investigation. These studies were approved by the Hospital de Navarra Ethics Committee. Production of proinflammatory Th1 cytokines (TNF‐alpha, IFN‐gamma, IL‐1B, IL‐6 and IL‐8) and anti‐inflammatory Th2 cytokines (IL‐10 and IL‐13)) was analyzed in mucosal biopsies of patients with UC (n=10) and control subjects (n=10) by flow cytometry using Cell Quest software (Becton Dickinson). The incubation of synbiotic reduced significantly (P< 0.01) the secretion of IL‐6, IL‐1beta and IL‐8 in biopsies of UC patients. The anti‐inflammatory Th2 cytokine levels were not affected either by UC disease or by synbiotic treatment. These findings suggest a new immunomodulatory function of this symbiotic compound and further suggests that these bacteria may be useful in the treatment of UC. This work has been funded by Ortiz de Landazuri of Navarra Government and Ministry of Education & Science (AGL2006‐10296).