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Diabetes alters the production and release of catecholamines in Primary Mesangial Cell Culture
Author(s) -
Cunha Tatiana S,
Arita Danielle Yuri,
Nogueira Marie Doki,
Perez Juliana Dinéia,
Andrade Maria Claudina Camargo,
Casarini Dulce Elena
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.971.8
Subject(s) - diabetes mellitus , medicine , endocrinology , streptozotocin , intracellular , extracellular , epinephrine , chemistry , biochemistry
Catecholamines (CA) are produced by mesangial cells (MC), and their levels are increased in diabetes. The aim was to asses the effect of diabetes duration on the production/release of CA‐dopamine (DA), epinephrine (EP) and norepinephrine (NE)‐in cultured MC. Male Wistar rats were randomized into Control, Diabetes 3M (3 months diabetes) and Diabetes 4M (4 months diabetes) groups. Rats were made diabetetic (streptozotocin, 50mg/Kg) and after 3 or 4 months, kidneys were excised for glomeruli isolation. MC were cultured in DMEM, CA were extracted and quantified by high performance liquid chromatography. Extracellular level of EP was increased in Diabetes 3M group, as compared to control group (55±9 vs. 29±3 pg/mg protein). On the other hand, 4 months of diabetes increased intracelular concentration of EP (77±10 pg/mg protein) and NE (76.5±10 pg/mg protein) compared with control and Diabetes 3M groups, with no effect on DA levels. Intracellular concentration of EP and NE was not affected after 3 months of diabetes; however DA level was significantly decreased in Diabetes 3M group (81±5 pg/mg), as compared to control (under detection limit). In Diabetes 4M group, we observed an increase in intracellular concentration of EP (151±27 pg/mg protein) and NE (53±14 pg/mg protein) and a decrease of DA levels. Our results show that diabetes duration influences the production/release of catecholamines by MC and suggests that this pathway can have a role on renal dysfunction induced by time‐duration of diabetes. Financial Support: CNPq.