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Influence of experimental early diabetic nephropathy on the functional subtypes of renal α1‐adrenoceptor in spontaneously hypertensive rats (SHR)
Author(s) -
Khan Md Abdul H,
Sattar Munavvar A,
Abdullah Nor A,
Majid Dewan S A,
Johns Edward J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.971.7
Subject(s) - medicine , endocrinology , creatinine , diabetic nephropathy , renal function , nephropathy , population , phenylephrine , diabetes mellitus , blood pressure , environmental health
This study investigated the impact of early diabetic nephropathy (EDN) on the functional population of renal α1‐adrenoceptors in SHR rats. One week after streptozotocin, EDN was confirmed by hyperglycemia (14.8±2.3 vs 5.7 ±0.6 mg/dL), raised creatinine clearance (4.2±0.10 vs 0.5±0.07 mL/min/100g), albumin‐creatinine ratio (94.6±3.7 vs 39.4±2.6 µg albumin/mg creatinine) and fractional excretion of Na + (1.2±0.01 vs 0.4±0.01 %) compared to non‐diabetic (NDN) rats. In acute studies, renal vasoconstrictions caused by renal nerve stimulation and intrarenal noradrenaline, phenylephrine and methoxamine were determined in the presence and absence of intrarenal amlodipine (AMP), 5‐methylurapidil (MEU), chloroethylclonidine (CEC) and BMY 7378. In both NDN and EDN rats, the renal vasoconstrictions were attenuated by all antagonists (AMP‐ NDN, 5‐15% and EDN, 8‐20%; MEU‐ NDN, 5‐14% and EDN, 9‐14%; BMY 7378‐ NDN, 5‐10% and EDN, 5‐8%). CEC enhanced these responses in EDN (5‐10%) but did not cause any effect in NDN SHR rats. The data showed that α1A and α1D are the renal functional α1‐adrenoceptors in SHR rat irrespective of the presence of EDN. A minor contribution of pre‐synaptic α1B‐adrenoceptors was observed in the EDN group.