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Restriction of dietary NaCl decreases urinary output in diabetic rats
Author(s) -
O'Neill Heidi,
Kwon TaeHwan,
Ring Troels,
Dimke Henrik,
Lebeck Janne,
Frøkiær Jørgen,
Collins Patrick B,
Nielsen Søren,
Frische Sebastian
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.971.11
Subject(s) - polyuria , medicine , endocrinology , streptozotocin , diabetes mellitus , urinary system , chemistry , zoology , biology
One week after induction of uncontrolled diabetes mellitus (DM) in female rats by streptozotocin injection, the effect of dietary NaCl restriction on urinary volume (U V ) was investigated. In the first experiment (n=6 in each group), rats were offered a synthetic NaCl‐deficient diet (C1036) for 2 days. In DM rats, U V increased dramatically (111±2 vs 211±7 ml/day; p<0.001), whereas non‐diabetic controls showed no change in U V (13.1±1.9 vs 11.7±1.5 ml/day; p=0.6). In the second experiment (n=5 in each group), DM rats receiving C1036 for 2 days again showed a dramatic increase in U V , however the increase and degree of polyuria were similar in DM rats receiving C1036 supplemented with NaCl to normal dietary levels (170±10 vs 174±7 ml/day; p=0.61). No changes were evident in non‐diabetic control rats receiving either diet. In the third experiment (n=8 in each group) standard rat chow (1320) or a custom‐made standard rat chow with reduced (32%) Na + content (1320‐Na + 32%), were given for 7 days. U V was lower in DM rats given 1320‐Na + 32% than in DM rats given standard 1320 chow (114±11 vs 151±7 ml/day; p=0.007). No difference was seen between non‐diabetic control groups receiving 1320‐Na + 32% or 1320. We conclude 1) administration of the synthetic chow, C1036, to DM rats increased U V dramatically irrespective of the NaCl content, and 2) without a concurrent change in the dietary background a reduction in dietary NaCl leads to decreased U V in DM rats.