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Adenoviral endothelial nitric oxide synthase (NOS) expression in the paraventricular nucleus (PVN) inhibits mean arterial blood pressure in renal wrap hypertension
Author(s) -
Northcott Carrie A,
Chen Alex F,
Haywood Joseph R
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.967.9
Subject(s) - enos , mean arterial pressure , medicine , blood pressure , endocrinology , nitric oxide synthase , nitric oxide , heart rate
PVN nitric oxide (NO) has been implicated in hypertension. Moreover, PVN neuronal NOS is reduced during the onset of renal wrap hypertension. Thus, we hypothesized that increasing PVN NO would reduce the increase in mean arterial pressure (MAP) observed in renal wrap hypertension. In male sprague dawley rats radiotelemetry transmitters were implanted for MAP and heart rate (HR) measures. Baseline MAP and HR were obtained for one week. Adenoviruses (Ad) encoding eNOS or lacZ (a marker gene/control) were then microinjected into the PVN [1 x 10 9 pfu; bilateral (200nl/site)]. After one week of MAP and HR baseline measures post PVN Ad injections, renal wrap/sham surgeries were performed. Neither Ad LacZ nor Ad eNOS alone had effects on MAP or HR. Renal wrap surgery significantly increased MAP in both Ad LacZ and Ad eNOS wrap animals compared to sham counterparts. Five days post surgery the Ad eNOS wrap animals had a significantly lower MAP compared to Ad LacZ wrap rats (Ad LacZ wrap: 137 + 4; Ad eNOS wrap: 124 + 9; Ad Lac Z sham: 104 + 3; Ad eNOS sham: 107 + 5; p < 0.05), with maximal inhibition occurring 10 days post wrap (Wrap Ad Lac Z: 146 + 6 vs. Ad eNOS: 123 + 12 mm Hg, p < 0.05). In conclusion, these data demonstrate that an increase in eNOS in the PVN, potentially increasing PVN NO, results in a blunting of MAP in renal wrap hypertension further demonstrating the role of PVN NO in blood pressure regulation in hypertension.