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Retinoic acid decreases endothelial permeability and fenestrae‐associated protein PV‐1 expression in human umbilical vein endothelial cells (HUVEC)
Author(s) -
Bodor Csaba,
Németh Adrienn,
Sebe Attila,
Rosivall László
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.965.17
Subject(s) - umbilical vein , permeability (electromagnetism) , endothelial stem cell , vascular endothelial growth factor , retinoic acid , human umbilical vein endothelial cell , vascular permeability , western blot , endocrinology , chemistry , biology , medicine , cell culture , in vitro , biochemistry , vegf receptors , membrane , gene , genetics
Vascular endothelial growth factor (VEGF), recognized as a growth factor secreted by tumor cells, increases PV‐1 expression and endothelial permeability. Retinoic acid (RA), a vitamin A derivative, regulates cell differentiation and is implicated in obesity, diabetes and cardiovascular disease. We postulated that RA may alter the effects of VEGF on HUVEC. To test this hypothesis we measured if administration of RA modulates PV‐1 expression and endothelial permeability in cell culture. HUVEC cultures were treated with 1 and 10 μM of RA. mRNA and protein levels of PV‐1 were detected with real‐time PCR and western blot, respectively. Permeability of endothelial monolayer was determined using 40 kDa FITC‐labeled dextran. 10 μM of RA significantly decreased the mRNA and protein level of PV‐1 and this effect was most pronounced after 48 hours. 10 μM of RA also decreased significantly the permeability of endothelial monolayer. Preincubation of HUVEC for 30 minutes with RA increased cell proliferation and blocked the endothelial permeability induced by 100 ng/ml VEGF. We demonstrated that RA decreased permeability and PV‐1 expression and blocked the effect of VEGF on endothelial permeability. Our results also suggest that RA may modulate the endothelial permeability effect of VEGF which at least partly induced by PV‐1 protein. Further studies needed to clarify the functional consequences of this striking phenomenon.