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Anatomic changes in the facial nucleus (FN) and surrounding area of goats after near total chronic destruction of the pre‐Botzinger complex (PBC) area
Author(s) -
Neumueller Suzanne,
Krause Katie,
Bonis Josh,
Qian Baogang,
Pan Lawrence,
Forster Hubert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.960.1
Subject(s) - anatomy , nissl body , biology , medulla , medicine , pathology , staining
We have reported normal breathing and arterial blood gases in awake and sleeping goats after step‐wise destruction of 80 to 92% of the PBC and surrounding area (FASEB J. 2008 22:755.16). To explore possible anatomic sites of plasticity following this destruction, the cryopreserved medulla of 4 PBC lesioned goats and 4 control goats were cyrosectioned (25ìm), Nissl stained, and then we quantified the area and number of neurons 2mm caudal and rostral to the caudal boundary of the FN and the area ventral and lateral to this 4mm area. The area (mm2) of the first 2mm of the FN was nearly 75% less (P<0.001) in PBC lesioned goats than in control goats. As a result, the total number of FN neurons was about 60% less (P<0.001) in lesioned than in control goats. The number of neurons for 1mm caudal to the FN did not differ between lesioned and control goats. Ventral to the FN and ventral to the 2mm caudal to the FN, there was no difference in the total area (P=0.291) between lesioned and control goats but throughout this area there were about 50% more (P<0.001) neurons in the lesioned than in control goats. Lateral to the FN and lateral to the 2mm caudal to the FN, there was no difference (P=0.405) in numbers of neurons between control and lesioned goats. We conclude that chronic lesions of the PBC result in anatomic change in the FN and areas caudal and ventral to the FN. Supported by NIH 25739 and the Department of Veterans Affairs.