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Insights into novel host factors required for HIV‐1 replication in human cells
Author(s) -
Jeang KuanTeh
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.96.2
Subject(s) - small hairpin rna , biology , virology , gene , viral replication , lentivirus , human immunodeficiency virus (hiv) , vector (molecular biology) , virus , replication (statistics) , computational biology , genetics , rna , recombinant dna , viral disease
Comprehensive genomewide screening of genes needed by various viruses for replication in human cells has become achievable with the advent of large libraries of siRNAs or shRNAs. To study genes needed for HIV‐1 to replicate in human T‐cells, a lentivirus‐vector based shRNA library that targets 47,400 human mRNAs using 200,000+ discrete shRNAs was employed. This approach allowed for the generation of independent cell clones, each chronically and individually expressing a single shRNA. When a pool of these cell clones was infected with high titer HIV‐1, cells that survived virus‐infection were ones that have knocked‐down a human mRNA whose loss interrupted productive HIV‐1 infection. The analyses of survivor cells revealed a set of candidate mRNAs that are needed for HIV‐1 replication. This approach and some of the gene candidates and their functions revealed from this study will be discussed.