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Increased oxidative stress in cardiovascular center of brain stem causes sympatho‐excitation in dietary‐induced obesity rat
Author(s) -
Konno Satomi,
Hirooka Yoshitaka,
Kishi Takuya,
Ito Koji,
Araki Shuuichiro
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.958.18
Subject(s) - rostral ventrolateral medulla , medicine , endocrinology , adiponectin , oxidative stress , leptin , tbars , blood pressure , obesity , metabolic syndrome , heart rate , lipid peroxidation , insulin resistance
Background Activation of the sympathetic nerve activity (SNA) induced by leptin acting on the pituitary‐hypothalamic pathway plays a central role of hypertension in metabolic syndrome (MetS). However, the role of the cardiovascular center in the brain stem (rostral ventrolateral medulla; RVLM) in SNA and the mechanism involved in MetS are not understood. The aim of this study was to determine if oxidative stress in the RVLM in MetS is increased thereby causing hypertension. Methods and Results Male Sprague‐Dawley rats were fed on a moderately high‐fat diet and segregated into obesity‐prone (OP, n=5) and obesity‐resistant (OR, n=5) groups based on their body weight (BW) after 13 weeks. Adiponectin in blood sample and visceral fatty mass were measured as an indicator of MetS. Blood pressure (BP), hearts rate (HR), urinary norepinephrine excretion (UNE), body weight, and amount of visceral fat mass were significantly higher and adiponectin was significantly lower in OP than in OR. Thiobarbituric acid‐reactive substances (TBARS) levels in the RVLM were significantly higher in OP than in OR. (7.2±0.8μmol/g wet wt vs 2.2±1.9μmol/g wet wt, n=3 for each, p<0.05). Conclusion Oxidative stress in the RVLM of OP was increased associated with BP, HR, and UNE elevation and the changes in MetS markers. Increased oxidative stress in the RVLM may increase SNA thereby leading to hypertension in MetS.