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Effects of inducible nitric oxide synthase (iNOS) blockade within the periaqueductal gray on cardiovascular responses during mechanical, heat, and cold nociception
Author(s) -
Ally Ahmmed,
Tedesco Anthony,
Chaitoff Kevin A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.956.6
Subject(s) - microdialysis , nociception , nitric oxide synthase , nitric oxide , periaqueductal gray , chemistry , stimulation , stimulus (psychology) , anesthesia , medicine , endocrinology , pharmacology , central nervous system , receptor , psychology , midbrain , psychotherapist
We examined the role of inducible nitric oxide synthase (iNOS) within the periaqueductal gray mater (PAG) on cardiovascular responses and glutamate and GABA concentrations during mechanical, thermal, and cold nociception in anesthetized rats. Mechanical stimulus was applied by bilateral hindpaw pinch (10 sec) that increased mean arterial pressure (MAP) and heart rate (HR). Bilateral microdialysis of a selective aminoguanidine (AGN; 10 μM) into the PAG for 30 min potentiated MAP and HR during a mechanical stimulation. Cardiovascular responses recovered following discontinuation of the drug. Heat stimulus was generated by immersing both hindpaw metatarsus in a water‐bath at 52°C for 10 sec. MAP and HR were increased, but administration of AGN into the PAG also augmented cardiovascular responses during the heat stimulus. Cardiovascular responses recovered following discontinuation of the drug. In contrast, application of a cold stimulus by immersing both hindpaw at 10°C for 10 sec resulted in decreases of MAP and HR. However, following microdialysis of AGN into the PAG, a cold stimulus had no effect on the cardiovascular responses. Results demonstrate that iNOS within the PAG plays a differential role in modulating cardiovascular responses during mechanical‐, heat‐, and cold‐mediated nociception.